基于线粒体12S基因分析四川地区鸡异刺线虫的种群遗传多态性

doi:10.11843/j.issn.0366-6964.2015.12.019

Abstract

Abstract:

To study the population genetic diversity and genetic structure of Heterakis gallinarum population in Sichuan，the complete sequence of mitochondrial 12S gene of 58 H.gallinarum isolates from 7 different geographical regions in Sichuan were amplified and analyzed the genetic diversity.The results showed that the complete lengths of mitochondrial 12S gene sequenced from 58 H.gallinarum isolates in this study were all 699 bp.These sequences contained 38 variable sites and were classified into 34 haplotypes(HS1-HS34).The values of haplotypes diversity(Hd) and nucleotide diversity(π) were 0.822 and 0.003 95，respectively.Genetic diversity with Fst and Nm furtherly showed that there were no significant genetic differentiation and frequent gene flow among the seven populations(Fst=0.007 87，Nm=31.52).An analysis of molecular variance(AMOVA) estimated that 99.21% of the genetic variation was partitioned within the population，and only 0.79% occurred among populations.NJ phylogenetic tree and haplotype network were both revealed that 34 haplotypes from 7 different geographical regions dispersed different geographical populations，and haplotypes did not cluster according to their geographical location.The results indicated that the H.gallinarum population in Sichuan had a low level of genetic diversity and no significant genetic differentiation，it have not formed significant geographical genetic structure.

CLC Number:

S852.731

WANG Bao-jian，ZHU Jun-yang，JIAN Ke-ling，YANG Guang-you，WANG Tao，LAI Wei-min，ZHAO Xi-bin，LIAO Xiao-xia，GU Xiao-bin. Population Genetic Polymorphisms of Heterakis gallinarum in Sichuan Base on Mitochondrial 12S Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2264-2272.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.12.019

https://www.xmsyxb.com/EN/Y2015/V46/I12/2264

References

［1］杨光友，张志和.野生动物寄生虫病学［M］.四川出版集团，2005：554-556.
YANG G Y，ZHANG Z H.Parasitic diseases of wildlife［M］.Sichuan Publishing Group，2005：554-556.(in Chinese)
［2］EBRAHIMI M,ASADPOUR M,KHODAVERDI M, et al.Prevalence and distribution of gastrointestinal helminthes in free range chickens in Mashhad，northeast of Iran［J］.Scientia Parasitologica，2014，15(1-4)：38-42.
［3］鞠宏生.鸡异刺线虫的流行情况及防治措施［J］.养殖技术顾问，2011(5)：129.
JU H S.The epidemiological and prevention of Heterakis gallinarum［J］.Technical Advisor for Animal Husbandry，2011(5)：129.(in Chinese)
［4］吕召宏，徐广庭，林瑞庆，等.鸡异刺线虫ITS rDNA的PCR扩增、克隆及序列分析［J］.中国畜牧兽医，2005，32(8)：41-44.
LV Z H，XU G T，LIN R Q，et al.PCR amplification，cloning and sequence analysis of ITS rDNA gene separated from Heterakis gallinarum ［J］.Chinese Animal Husbandry and Veterinary Medicine，2005，32(8)：41-44.(in Chinese)
［5］MWALE M，MASIKA P J.In vivo anthelmintic efficacy of Aloe ferox，Agave sisalana，and Gunnera perpensa in village chickens naturally infected with Heterakis gallinarum［J］.Trop Anim Health Prod，2015，47(1)：131-138.
［6］张鹏.鸡组织滴虫病与异刺线虫病混合感染的诊治［J］.中国家禽，2012，34(17)：50-51.
ZHANG P.Diagnosis and Treatment of Chicken Histomoniasis and Heterakis gallinarum mixed infection［J］.China Poultry，2012，34(17)：50-51.(in Chinese)
［7］SCHWARZ A，GAULY M，ABEL H，et al.Pathobiology of Heterakis gallinarum mono-infection and co-infection with Histomonas meleagridis in layer chickens［J］.Avian Pathol，2011，40(3)：277-287.
［8］BAZH E K.Molecular identification and phylogenetic analysis of Heterakis gallinae from native chickens in Egypt［J］.Parasitol Res，2013，112(10)：3557-3560.
［9］JIMÉNEZ F A，GARDNER S L，NAVONE G，et al.Four events of host switching in Aspidoderidae(Nematoda) involve convergent lineages of mammals［J］.J Parasitol，2012，98(6)：1166-1175.
［10］延宁，聂华明，蒋忠荣，等.基于线粒体 12S 基因对青海地区细粒棘球蚴种群遗传多态性研究［J］.畜牧兽医学报，2012，43(6)：972-978.
YAN N，NIE H M，JIANG Z R，et al.Genetic polymorphisms of Echinococcus granulosus in Qinghai based on the mitochondrial DNA 12S gene［J］. Acta Veterinaria et Zootechnica Sinica，2012，43(6)：972-978.(in Chinese)
［11］徐梅英，李继姬，郭宝英，等.基于线粒体 DNA 12S rRNA 和 COⅢ基因序列研究中国沿海 7个长蛸(Octopus variabilis) 野生群体的遗传多样性［J］.海洋与湖沼，2011，42(3)：387-396.
XU M Y，LI J J，GUO B Y，et al.Genetic diversity of seven populations of Octopus Variabilis in China’s coastal waters based on the 12S rRNA and COⅢ gene analysis［J］.Oceanologia et Limnologia Sinica，2011，42(3)：387-396.(in Chinese)
［12］MOVILA A，MOROZOV A，SITNICOVA N.Genetic polymorphism of 12S rRNA gene among Dermacentor reticulatus fabricius ticks in the chernobyl nuclear power plant exclusion zone［J］.J Parasitol，2013，99(1)：40-43.
［13］ZHOU X，XIE Y，ZHANG Z H，et al.Analysis of the genetic diversity of the nematode parasite Baylisascaris schroederi from wild giant pandas in different mountain ranges in China［J］.Parasit Vectors，2013，6：233.
［14］张路平.动植物寄生线虫的群体遗传结构研究进展［DB/OL］.中国科技论文在线精品论文，2011，17(4)：1532-1536.
ZHANG L P.Research progress on population genetic structure of parasitic nematodes from animal and plant［DB/OL］.Highlights of Sciencepaper Online，2011，17(4)：1532-1536.(in Chinese)
［15］彭俊宇，宋海燕，陈文承，等.湖南省部分地区商品鸡异刺线虫感染情况调查［J］.中国动物传染病学报，2009，17(1)：51-53.
PENG J Y，SONG H Y，CHEN W C，et al.Prevalence of Heterakis in chickens in Hunan province［J］.Chinese Journal of Animal Infectious Diseases，2009，17(1)：51-53.(in Chinese)
［16］SAMBROOK J，RUSSELL D W.Molecular cloning［M］.New York：Cold Spring Harbor Laboratory Press，1989.
［17］TAMURA K，PETERSON D，PETERSON N，et al.MEGA5：molecular evolutionary genetics analysis using maximum likelihood，evolutionary distance，and maximum parsimony methods［J］.Mol Biol Evol，2011，28(10)：2731-2739.
［18］LIBRADO P，ROZAS J.DNAsp v5：software for comprehensive analysis of DNA polymorphism data［J］.Bioinformatics，2009，25(11)：1451-1452.
［19］CLEMENT M，POSADA D，CRANDALL K A.TCS：a computer program to estimate gene genealogies［J］.Mol Ecol，2000，9(10)：1657-1659.
［20］EXCOFFIER L，LISCHER H E.Arlequin suite ver 3.5：a new series of programs to perform population genetics analyses under Linux and Windows［J］.Mol Ecol Resour，2010，10(3)：564-567.
［21］BARRETT L G，THRALL P H，BURDON J J，et al.Life history determines genetic structure and evolutionary potential of host-parasite interactions［J］.Trends Ecol Evol，2008，23(12)：678-685.
［22］SU B，FU Y，WANG Y，et al.Genetic diversity and population history of the red panda(Ailurus fulgens) as inferred from mitochondrial DNA sequence variations［J］.Mol Biol Evol，2001，18(6)：1070-1076.
［23］杨金权，胡雪莲，唐文乔，等.长江口邻近水域刀鲚的线粒体控制区序列变异与遗传多样性［J］.动物学杂志，2008，43(1)：8-15.
YANG J Q，HU X L，TANG W Q，et al.mtDNA control region sequence variation and genetic diversity of Coilia nasus in Yangtze River Estuary and its adjacent waters［J］.Chinese Journal of Zoology，2008，43(1)：8-15.(in Chinese)
［24］郝桂英，杨应东，周英姿，等.基于线粒体cox1和Cyt b基因对四川地区多头带绦虫的种群遗传多样性研究［J］.畜牧兽医学报，2014，45(4)：631-638.
HAO G Y，YANG Y D，ZHOU Y Z，et al.Population genetic diversity of Taenia multiceps isolated from Sichuan based on Mitochondrial cox1 and cyt b gene［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(4)：631-638.(in Chinese)
［25］AVISE J C.Phylogeography：the history and formation of species［M］.Harvard University Press，2000：447-448.
［26］GRANT W S，BOWEN B W.Shallow population histories in deep evolutionary lineages of marine fishes：insights from sardines and anchovies and lessons for conservation［J］.J Hered，1998，89(5)：415-426.
［27］LAVERY S C，MORTIZ C，FIELDER D R.Indo-Pacific population structure and evolutionary history of the coconut crab Birgus latro［J］.Mol Ecol，1996，5(4)：557-570.
［28］HOLSINGER K E，WEIR B S.Genetics in geographically structured populations：defining，estimating and interpreting FST［J］.Nat Rev Genet，2009，10(9)：639-650.
［29］BLOUIN M S，YOWELL C A，COURTNEY C H，et al.Host movement and the genetic structure of populations of parasitic nematodes［J］.Genetics，1995，141(3)：1007-1014.
［30］TROUT R T，STELLMAN C D，SZALANSKI A L.Population genetics of Amblyomma americanum(Acari：Ixodidae) collected from Arkansas［J］.J Med Entomol，2010，47(2)：152-161.
［31］ARAYA-ANCHETTA A，BUSCH J D，SCOLES G A，et al.Thirty years of tick population genetics：A comprehensive review［J］.Infect Genet Evol，2015，29：164-179.
［32］POSADA D，CRANDALL K A.Intraspecific gene genealogies：trees grafting into networks［J］.Trends Ecol Evol，2001，16(1)：37-45.
［33］余文华.海南岛两种蝙蝠种群形态特征与地理亲缘关系的研究［D］.海口：海南师范大学，2009.
YU W H.Study on the morphologogy and phylogeography of two vespertilionid bats in Hainan［D］.Haikou：Hainan Normal University，2009.(in Chinese)

[1]	BO Luqi, WANG Xueying, HOU Jiaming, ZHANG Li, ZHANG Jianhui, RUAN Rulin, ZHANG Bohan, WANG Shuang, SONG Mingxin, ZHANG Ziqun. Interference of Ts-ODC Gene of Trichinella spiralis by siRNA-757 Effect on Its Acid Resistance [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2114-2125.
[2]	WANG Bingnan, SUN Xue, JIA Hongguo, TAN Chun, MEI Sipeng, ZHOU Rongqiong. Prokaryotic Expression and Tissue Localization of C-type Lectin 4 from Toxocara canis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1641-1651.
[3]	LIANG Gaoxing, RONG Shiqi, WANG Junwei, LI Yuan, YANG Xin, ZHAO Guanghui, SONG Junke. Multi-locus Gene Sequence Analysis of Female Haemonchus contortus with Morphological Differences in Vulval Flap from Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3140-3148.
[4]	LIAO Shuyi, CHU Wen, XU Lixin, SONG Xiaokai, LI Xiangrui, YAN Ruofeng. Effect of Trichinella spiralis Recombinant Dipeptidyl-peptidase 1 on Proliferation, Phagocytosis, and Apoptosis of Rat PBMCs in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1362-1370.
[5]	CHEN Shaoji, LI Fang, YIN Shasha, JIANG Aiyun, ZHOU Rongqiong. Expression and Chitin-Binding Feature of TcCPG1 of Toxocara canis [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 356-364.
[6]	LUO Yongli, JIANG Aiyun, HU Zhigang, LI Xianglan, YANG Xiaodi, KUANG Ceyan, ZHOU Rongqiong. Effect of RNA Interference abcg-5 Gene on the Infectivity of Toxocara canis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 390-397.
[7]	WU Yiran, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 151-158.
[8]	ZHOU Li-na, HU Meng-juan, SUN Wei, LI Xiang-rui, XU Li-xin, SONG Xiao-kai, YAN Ruo-feng. Gene Cloning and Biological Characteristics of Lipase Gene from Haemonchus contortus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 580-587.
[9]	WANG Wen-long, ZHAO Xue-liang, SUN Ke, FENG Chen-chen, BAI Li-yan, WANG Shu-yi, WANG Meng-ya, LIU Chun-xia. Cloning, Protein Structure Analysis and Antigenic Epitopes Prediction of Immune-related Gene, CAMK/TSSK Protein Kinase Gene of Parabronema skrjabini [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 644-651.
[10]	LIAO Cheng-shui, WANG Xiao-li, TIAN Wen-jing, YANG Ya-dong, ZHANG Meng-ke, WANG An-qi, ZHANG Chun-jie, LIU Ming-yuan, BOIREAU Pascal, CHENG Xiang-chao. Cloning and Sequence Analysis of Plancitoxin-1-like Gene in Trichinella spiralis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 218-222.
[11]	WU Ling-yan, WANG Yu-jian, WEN Yu-ling, YAN Ruo-feng, XU Li-xin, SONG Xiao-kai, LI Xiang-rui. Cloning, Expression and Function Analysis of NADH: Ubiquinone Oxidoreductase Domain Containing Protein in Haemonchus contortus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 722-730.
[12]	ZHENG You-le，WANG Cheng-dong，WANG Tao. Research Advances on Chemosensory Behaviors of Nematodes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 857-863.
[13]	GU Xiao-bin，ZHU Jun-yang，WANG Bao-jian，ZHENG Jing，YANG Guang-you，WANG Tao，LAI Wei-min. Genetic Variation of Heterakis gallinarum in Sichuan based on the Nuclear Ribosomal DNA Internal Transcribed Spacer Regions (ITS1/2) [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 796-804.
[14]	MA Guang-xu，ZHU Hong-hong，HU Ling，LI Wei-lin，LIN Jie，ZHOU Rong-qiong. Differential Expression of Gender-related Genes Tc-vit，Tc-iom，Tc-tpk，Tc-stp in the Germ-line Tissues of Toxocara canis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(3): 461-466.

Population Genetic Polymorphisms of Heterakis gallinarum in Sichuan Base on Mitochondrial 12S Gene

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments